https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064141/
ABSTRACT
Feline leukemia virus (FeLV) induces neoplastic and nonneoplastic diseases in cats. The transduction of cellular genes by FeLV is sometimes observed and associated with neoplastic diseases including lymphoma and sarcoma. Here, we report the first natural case of feline Notch2 transduction by FeLV in an infected cat with multicentric lymphoma and hypercalcemia. We cloned recombinant FeLVs harboring Notch2 in the env gene. Notch2 was able to activate expression of a reporter gene, similar to what was previously reported in cats with experimental FeLV-induced thymic lymphoma. Our findings suggest that the transduction of Notch2 strongly correlates with FeLV-induced lymphoma.
Expression and activation of v-Notch2 protein. (A) Expression of Myc-tagged v-Notch2 proteins in transiently transfected HEK293T cells. HEK293T cells were transfected with pFUΔss expression vector (vector) [1] or pFUΔss-KeyN2-Myc (v-N2-Myc). Cells were collected after 48 hr, and total cell lysates were subjected to Western blotting analysis using mouse anti-Myc (Wako, Osaka, Japan) or mouse anti-β-Actin antibody (Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.). (B) HEK293T cells in 24-well plate were co-transfected with pGa981-6 (50 ng), phRL-CMV (5 ng) and v-Notch2 expressing plasmids (v-N2). Luciferase assay was performed in triplicate, and the relative luciferase activity was measured using Dual-Luciferase Reporter Assay System (Promega) at 48 hr post transfection. The relative luciferase unit (RLU) is shown relative to the negative control (vector). Error bars denote the standard deviation (SD). **P<0.01 using unpaired t-test.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064141/
ABSTRACT
Feline leukemia virus (FeLV) induces neoplastic and nonneoplastic diseases in cats. The transduction of cellular genes by FeLV is sometimes observed and associated with neoplastic diseases including lymphoma and sarcoma. Here, we report the first natural case of feline Notch2 transduction by FeLV in an infected cat with multicentric lymphoma and hypercalcemia. We cloned recombinant FeLVs harboring Notch2 in the env gene. Notch2 was able to activate expression of a reporter gene, similar to what was previously reported in cats with experimental FeLV-induced thymic lymphoma. Our findings suggest that the transduction of Notch2 strongly correlates with FeLV-induced lymphoma.
Expression and activation of v-Notch2 protein. (A) Expression of Myc-tagged v-Notch2 proteins in transiently transfected HEK293T cells. HEK293T cells were transfected with pFUΔss expression vector (vector) [1] or pFUΔss-KeyN2-Myc (v-N2-Myc). Cells were collected after 48 hr, and total cell lysates were subjected to Western blotting analysis using mouse anti-Myc (Wako, Osaka, Japan) or mouse anti-β-Actin antibody (Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.). (B) HEK293T cells in 24-well plate were co-transfected with pGa981-6 (50 ng), phRL-CMV (5 ng) and v-Notch2 expressing plasmids (v-N2). Luciferase assay was performed in triplicate, and the relative luciferase activity was measured using Dual-Luciferase Reporter Assay System (Promega) at 48 hr post transfection. The relative luciferase unit (RLU) is shown relative to the negative control (vector). Error bars denote the standard deviation (SD). **P<0.01 using unpaired t-test.