Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

Keith Pardee et al. Cell, Volume 165, Issue 5, p1255–1266, 19 May 2016

Highlights

  • A portable, low-cost diagnostic platform for the detection of Zika virus
  • Discrimination of viral strains at single-base resolution using a CRISPR-based tool
  • Low femtomolar detection of Zika virus from infected monkey plasma
  • Programmable sensor development workflow for rapid responses to global epidemics 

Summary


The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, our sensors can discriminate between viral strains with single-base resolution. We successfully demonstrate a simple, field-ready sample-processing workflow and detect Zika virus from the plasma of a viremic macaque. Our freeze-dried biomolecular platform resolves important practical limitations to the deployment of molecular diagnostics in the field and demonstrates how synthetic biology can be used to develop diagnostic tools for confronting global health crises.

CytokinesHuman Mannan-Binding Lectin Serine Protease 1/MASP1 / C655

Catalog#C655
SourceHEK293
DescriptionRecombinant Human Mannan-Binding Lectin Serine Protease 1/MASP1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (His20-Arg728) of Human MASP1 fused with a polyhistidine tag at the C-terminus.
NamesMannan-Binding Lectin Serine Protease 1, Complement Factor MASP-3, Complement-Activating Component of Ra-Reactive Factor, Mannose-Binding Lectin-Associated Serine Protease 1, MASP-1, Mannose-Binding Protein-Associated Serine Protease, Ra-Reactive Factor S
Accession #P48740
FormulationSupplied as a 0.2
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